High-throughput sequencing

We use two platforms for high-throughput sequencing: From Life Technologies we use the SOLiD4 and 5500XL platforms.
Please contact Joris Veltman for collaboration possibilities.

Life Technologies SOLiD

With the Life Technologies systems we are identifying disease causing variants for rare mendelian disorders and intellectual disability. We currently perform exome- and RNA sequencing, which we perform on both the Solid 4 and 5500XL systems.




Roche 454 Sequencing

With the Roche 454 FLX Titanium we perform targeted resequencing to find causative variants within heterogeneous diseases, such as hereditary blindness. Multiple genes can easily be screened in a large cohort of patients to identify the disease causing variants.



Microarray platforms

We have different types of microarray platforms at our disposal to perform genomic profiling, homozygosity mapping, and targeted enrichment for high-throughput sequencing. Please contact Joris Veltman for collaboration possibilities.


Hybridisation station, which is used for targeted sequencing


Bioinformatics


The bioinformatics room.

Currently in our bioinformatics group, we have 2 separate computing clusters, one for backup and testing (7 nodes + 1 headnode, 8 CPUs, 24GB RAM each, with 18Tb storage) and a second cluster for production (10 nodes + 1 headnode, 48GB of RAM, with 18Tb storage). Additionally we have an 18Tb storage server for sharing results within the department, as well as a server hosting a local copy of the UCSC browser.


Wall-E, computer cluster used for the
454 Sequencing data analysis
Server rack, contains the following servers:
Scrat, Fiona, Sully, Crush, Russel


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